Free Online lectures on Biochemistry for Medical & Allied Health Sciences students by Biochemistry Club
- Knoop 1904 was the first scientist to discover β-oxidation.
- The carbon that is attached to the carboxylic group is known as α-carbon, the carbon next to it is known as β- carbon (3rd carbon including the COOH group). Oxidation of fatty acids occurs at the β-carbon. That is why it is known as β- oxidation. The last carbon is known as omega carbon.
- When fats are hydrolyzed by lipases, we get glycerol plus fatty acids.
- Glycerol comes to the liver where by enzyme glycerol kinase it will form glycerol-3-PO4, which can be dehydrogenated by Glycerol -3- PO4 dehydrogenase to DHAP- intermediate of glycolysis can be used for energy purpose or for gluconeogenesis by reversal of glycolysis. Secondly the Glycerol-3 PO4 can go in the adipose tissue.
- For the synthesis of fat glycerol must be in Glycerol -3- PO4 form. Adipose tissue does not contain glycerol kinase so they are dependent on liver for the supply of Glycerol -3- PO4.
- Fatty acids are known as free fatty acids or NEFA. These FFA are bound to albumin in plasma for transport and in the cells to FA binding protein (never free).
- Beta oxidation takes place in mitochondria while FFA are present in cytosol. These FA have to be transferred into the mitochondria for oxidation.
Steps of Beta-Oxidation
- Oxidation – removal of Hydrogen or addition of oxygen (FAD dependent dehydrogenation)
- 2nd oxidation (NAD dependent)
FOR COMPLETE LECTURE PLEASE VISIT THE COURSE PAGE IN BIOCHEMISTRY II SECTION. CLICK THE CURRICULUM TAB TO VIEW FULL LIST IN METABOLISM OF LIPIDS.